TCBS Selection Agar Limitations

TCBS Selection Agar Limitations

Shrimp farms can be stressful environments that have high organic matter and experience fluctuations of dissolved oxygen.  Changes in the environment in the shrimp farm will influence the number of bacteria and the species present.  For example, as the temperature increases and the salinity increases due to evaporation V. parahaemolyticus will thrive and become predominate. Shrimp farmers recognize the relevance of Vibrio species, and the safety level of heterotrophic bacteria and Vibrio to the health of their ponds.  Relative counts of bacterial groups are monitored and routine determination of Vibrio species are used to help in pond management.  Farmers realize that a high prevalence of green colonies on Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS) are indicative of diseased ponds. However, not all farmers may be aware of the pitfalls of the TCBS selection system or the availability of the improved HiCrome chromogenic Vibrio detection system.

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Prospecting for Probiotics

Prospecting for Probiotics

Most natural product drugs are secondary metabolites isolated from plants, fungi, and microorganisms. Penicillin is an example of a natural product produced by mold.  Although chemists have synthesized potential drugs, natural product drugs remain of interest. Initially microbes were isolated from soils and screened for useful metabolites.  As the quest continues for new drugs and new applications scientists have turned to the marine environment.  The ocean provides a plethora of diverse microbes able to grow in the warm water of the tropics or the cold waters of the arctic.

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KI Nitrifier – Nitrifying Oxidizing Ammonia

KI Nitrifier – Nitrifying Oxidizing Ammonia

Results from both the tank and RWs studies indicate that KI-Nitrifier was beneficial for accelerating the development of nitrifying bacteria and limiting the accumulation of nitrite to toxic concentrations. The dosage rate (50-200% of the recommended dose) made no significant difference to nitrite concentrations. The low temperatures experienced during both trials may have slowed the development of nitrifying bacteria.

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